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A strong correlation was observed between these pathways and the top Gene Ontology (GO) pathways, with the highest enrichment scores for GLUT1 (Fig

We selected pAcF as the handles for constructing conjugates instead of other chemistry such as azide mediated click chemistry as the cyclopropane-tetrazine ligation employed in the pAcF system demonstrates superior bioorthogonality compared to azide-alkyne cycloadditions, effectively minimizing off-target reactions in complex cellular environments